This study documents the first records of three rare spider species from the Republic of Moldova: Liocranoeca spasskyi Ponomarev, 2007 (Liocranidae), Marinarozelotes manytchensis (Ponomarev & Tsvetkov, 2006) (Gnaphosidae), and Oecobius rhodiensis Kritscher, 1966 (Oecobiidae). Specimens were collected in Chisinau during 2023–2024 through manual sampling and pitfall trapping at three sites. The findings substantially extend the known distribution ranges of the species. The record of O. rhodiensis represents the first national record of the family Oecobiidae in Moldova. Ecological data, including microhabitat descriptions and meteorological parameters, are provided for each site. Records of mature specimens also extend the known phenological periods for these species. The results contribute to the faunistic knowledge of the region and highlight Moldova’s potential as a biogeographic corridor for Eastern European spider fauna.