<p>The increasing threat of silver-resistance in clinical isolates from wounds and burns</p>

The widespread use of silver-containing compounds has led to emergence of silver-resistant bacteria. Few studies are available on the detectability of plasmid-mediated silver-resistance in developing countries. Therefore, we aimed to detect silver-resistance in isolates from wounds and burns, and to genetically characterize plasmid-mediated silver-resistance genes ( sil genes).

<p>Co-administration of aspirin and adipose-derived stem cell conditioned medium improves the functional recovery of the optic pathway in a lysolecithin-induced demyelination model</p>

Based on beneficial effects of aspirin and mesenchymal stem cells (MSCs) on myelin repair, in a preset study, effects of co-administration of aspirin and conditioned medium from adipose tissue-derived stem cells (ADSC-CM) on functional recovery of optic pathway, demyelination levels, and astrocytes' activation were evaluated in a lysolecithin (LPC)-induced demyelination model of optic chiasm.

EXPRESSION OF ARGYROPHILIC NUCLEOLAR ORGANIZING REGION COUNTS AND PROLIFERATING CELL NUCLEAR ANTIGEN IN EPITHELIAL ODONTOGENIC TUMORS

Odontogenic tumors are diverse groups of lesions derived from odontogenic tissues. Clinical behavior of these lesions varies widely, ranging from benign to malignant with differences in recurrence rate and growth pattern. A better understanding of the underlying molecular mechanisms will help to predict the course of odontogenic tumors and lead to the development of new therapeutic applications. Nucleolar organizer regions (NORs) are loops of DNA that codify the ribosomal RNA and are considered important for protein synthesis. The NORs stained histochemicaly by silver are named argyrophilic nucleolar organizer regions (AgNOR). Quantification of AgNOR represents a valuable parameter of cell kinetics and therefore cell proliferation. The number of AgNOR sites in malignant cells is significantly greater than those in their normal or benign counterparts. Proliferating cell nuclear antigen (PCNA) is a useful marker of cellular proliferation and DNA replication. Malignant tissue is characterized by an uncoordinated proliferating cell nuclear antigen (PCNA). OBJECTIVES: To evaluate the cell proliferation rate using the expression of Argyrophilic Organizing Region counts and Proliferating Cell Nuclear Antigen in epithelial odontogenic tumors and to assess the usefulness of AgNOR and PCNA as markers in malignant potential of epithelial odontogenic tumors. MATERIALS AND METHODS: This study was done on 45 surgical specimens of epithelial odontogenic tumors and one control specimen from normal tooth germ.The tissue biopsies were processed and paraffin sections were prepared. Hematoxylin and eosin-stained sections were examined for diagnosis. Other sections were subjected to AgNORhistochemical technique and immunohistochemical staining for PCNA. Immunohistochemical staining was performed using a Labeled Strept-Avidin Biotin complex method (LSAB). RESULTS: The mean AgNOR count of tooth germ was 1.21 and the benign odontogenic tumors was 1.862.The highest AgNOR count was recorded in Ameloblastic carcinoma and lowest was seen in adenomatoid odontogenic tumor. Statistically significant difference in AgNOR counts of ameloblastoma and adenomatoid odontogenic tumor, ameloblastoma and calcifying epithelial odontogenic tumor, benign odontogenic tumors and ameloblastic carcinoma were seen. AgNORs in ameloblastic carcinoma were more in number and more widely distributed.The highest mean value of PCNA-positive cell percentage was demonstrated in ameloblastic carcinoma followed by ameloblastoma. A significant difference was noted when the PCNA value of ameloblastoma was compared with that of ameloblastic carcinoma. CONCLUSIONS: AgNOR technique may be considered a good indicator of cell proliferation in epithelial odontogenic tumors.The PCNA expression, along with the clinical features, can predict the aggressiveness, chances of recurrence and malignant potential of these tumors.

THE EFFECT OF TOPICALLY APPLIED HYALURONIC ACID GEL VERSUS TOPICAL CORTICOSTEROID IN THE TREATMENT OF EROSIVE ORAL LICHEN PLANUS

Oral lichen planus is a common chronic inflammatory muco-cutaneous disease. Although the cause of oral lichen planus is not well known, T-lymphocyte infiltrate suggests a cell mediated immunological damage to basal cell layer of the epithelium. Its suggested treatment is topical corticosteroid. A promising new treatment for oral lichen planus [OLP] is the topical application of hyaluronic acid [HA], which has shown beneficial effect on wound healing. OBJECTIVES: to evaluate the effect of hyaluronic acid gel in the treatment of erosive oral lichen planus in comparison with topically applied corticosteroids. MATERIALS AND METHODS: a randomized, parallel, controlled clinical study was conducted on twenty patients who were randomly divided into two groups. Group I received topical application of corticosteroid (triamcinolone acetonide) 4-5 daily, for 28 days and Group II was similarly treated using hyaluronic acid 0.2% gel. Subjective symptom and Visual Analogue Scale (VAS) were recorded at base line, after 10 days and at 3 months. The objective sign clinical score was recorded at baseline, 1 and 3 months post –treatment. RESULTS: Both groups showed decrease in the lesion size and symptoms of OLP. The HA treated group showed a significantly higher decrease in the pain score in comparison to the corticosteroid treated group. Regarding the healing score, the corticosteroid group showed superior results than that shown with the HA. CONCLUSIONS: HA 0.2% gel is effective in the treatment of OLP, it resulted in a decrease in symptoms and signs of the disease. HA resulted in more control of pain when compared to topical corticosteroid.

IMMUNO- MORPHOLOGICAL PATTERNS OF LYMPH NODES DRAINING ORAL SQUAMOUS CELL CARCINOMA

Head and neck cancer (HNC) is the sixth most common cancer with 500,000 cases diagnosed per year worldwide. Oral squamous cell carcinoma (OSCC) is accounting for more than 90% of total cases. Among all factors influencing the prognosis of OSCC, development of lymphatic metastasis is considered the single most important one. Morphological evaluation of the reactive patterns of regional nodes has aided in understanding the immune response to malignancy. The World Health Organization (WHO) categorized this reactive pattern into lymphocytic predominance, germinal center predominance, sinus histiocytosis, unstimulated pattern and lymphocytic depletion. Assessment of lymph nodes immuno- reactivity is an important indicator of the host response status and prognosis of OSCC. OBJECTIVES: To Assess the immuno-morphological patterns of both positive and negative lymph nodes draining OSCC and correlate these patterns with the clinical finding and the histological grading of the primary tumor. Material and Methods: Histopathological examination had been done on 30 cases of OSCC with neck dissection and immuno-morphological patterns had been detected and correlated with the grading of primary tumor. Results: A statistical analysis of the distribution of the 5 patterns of lymph nodes in 3 grades of OSCC was done using chi square test. Significance difference was revealed only in two patterns (lymphocytic depletion and normal unstimulated patterns) .other 3 patterns was found to be non-significant. CONCLUSIONS: lymphocytic depletion and unstimulated mixed patterns of draining lymph nodes was significantly correlated with the grade of the tumor and it could be used as an indicator for prognosis and early metastasis of OSCC. However, no correlation was found between the other patterns and the histological grading.

The Role of Porphoromonas Gingivalis in Rheumatoid Arthritis and Periodontitis by Using Subantimicrobial Dose Doxycycline

Purpose: The current study was directed to estimate the role of porphoromonas gingivalis in the relation between rheumatoid arthritis and periodontitis by using subantimicrobial dose doxycycline.Subjects and methodology: The current study was accomplished on twenty patients who had mild or moderate chronic periodontitis and rheumatoid arthritis they were partitioned into two groups: Group I; ten patients were exposed to mechanical debridement only and Group II: ten patients were exposed to mechanical debridement added to receiving small dose doxycycline (SDD) as a host modulatory agent (20mg twice daily) for three months. Results: group I exhibited a greatly significant improvement in clinical parameter in correlation to group II after three months follow up. Group II showed the statistically significantly lowest percentage decrease in number of bacteria in synovial fluid Conclusion: The adjunct use of doxycycline as host modulatory agent improved the treatment outcomes when used with mechanical debridement in rheumatoid arthritis and patients with periodontitis.KEYWORDS: Rheumatoid Arthritis, Periodontitis, low dose doxycycline, Porphoromonas Gingivalis

Effect of vitamin A supplementation on performance and blood constituents of Saidi ewes and their offspring.

Forty pregnant ewes of more than two successive lambing seasons, at the second half of gestation period, were used to evaluate the effects of supplemented vitamin A on growth performance, blood constituents, levels of vitamin A and β˗carotene of Saidi ewes and their offspring. Pregnant ewes (48.48 ± 0.09 kg B. W.) were assigned to 4 treatment groups of 10 ewes each in the Farm of Animal Production Department, the Faculty of Agriculture, Assiut University, Assiut, Egypt.. Vitamin A was drenched in the form of pale yellow to brown granular powder as vitamin A acetate content ≥ 1,000,000 IU/g which had been certified by china council for the promotion of international trade.

Extraction of Some Rare Earth Elements (La, Pr and Er) from Citrate Medium Using D2EHPA in kerosene.

DETECTION OF MICROORGANISMS (BACTERIA, FUNGI AND YEASTS) IN ROYAL JELLY

The aim of the present study to detect the population and frequency (%) of microorganism (bacteria, fungi and yeasts) in royal jelly samples. The data indicated that, there are no significant differences were remarked in the population of microorganisms between all the samples for bacteria, fungi and yeasts, where the mean number of population was 5.923, 1.38 and 7.85 colonies/sample for bacteria, fungi and yeasts respectively, in produced royal jelly from honeybee colonies, local royal jelly collected from Egyptian market and samples of imported royal jelly collected from Egyptian market, respectively. According to the isolation and identification procedures for detected royal jelly samples, four bacteria types (Clostridium botulinum, Bacillus cereus, Bacillus wakoensis and Micrococcus luteus), two fungi types (Aspergillusniger and Penicillium sp.) and one yeast type (Saccharomyces cerevisiae) were determined. The data also summarized that Clostridium botulinum was the most frequency compared with the other bacterial types, where the percentage of frequency was 1.8 – 2.5, 0.9 – 1.4, 0.4 – 0.6 and 1.5 – 2.0% for C. botulinum, B. cereus, B. wakoensis and Micrococcus luteus, respectively. Meanwhile, Penicillium sp. the most frequency compared with A. niger, where the percentage of frequency was 0.5 - 2.9 and 0.7 – 1.0 %, respectively, in produced royal jelly from honeybee colonies, local royal jelly samples collected from Egyptian market and imported royal jelly samples collected from Egyptian market respectively. Regarding the yeasts, the data also summarized that, S. cerevisiae was the most frequency in royal jelly that produced from  honeybee colonies (2.9%) followed by which local royal jelly samples collected from Egyptian market (2.1%) and imported royal jelly samples collected from Egyptian market (1.8%).

EFFECT OF SPIRULIN (ARTHROSPIRA PLATENSIS) A AND NANNOCHLOROPSIS (NANNOCHLOROPSIS GADITANA) SUPPLEMENTATION ON GROWTH PERFORMANCE, FEED UTILIZATION AND CARCASS COMPOSITION OF NILE TILAPIA (Oreochromis niloticus)

The study was carried out  at  Faculty of Agriculture, Ain Shams University to assess the effect of two of algae species (Arthrospira platensis and Nannochloropsis gaditana) on growth performance of monosex  tilapia fish (Oreochromis niloticus), fish were fed on a basal diet supplemented with 0,3,5,7% of each algae. To perform seven experimental treatments were assigned in three replicates each. The experiment was designated as follows:  (T1) control (without algae), (T2) basal diet supplemented with 3% spirulina (spiru 3), (T3) basal diet supplemented with 5% spirulina (spiru 5), (T4) basal diet supplemented with 7% spirulina (spiru 7), (T5) basal diet supplemented with 3% Nannochloropsis (nanno 3), (T6) basal diet supplemented with 5% Nannochloropsis (nanno 5) and (T7) basal diet supplemented with 7% Nannochloropsis  (nanno 7). Experimental tanks were a part of closed recirculating system, where almost constant environmental conditions were kept throughout the experimental period. Twenty-one quadrate fiber glass tanks with 108-liter water capacity were stocked with 15 fish per tank. The individual initial body weight (2.7 g) was recorded at the beginning of the experiment. All fish in each tank was weighed every two weeks during the whole experimental period. The fish were fed the experimental diet for 95 days Results indicated that nanno 5 treatment had higher significant trend (P<0.05) final body weight (FBW), average weight  gain (AWG) and average daily gain (ADG) compared with the other experimental treatments. The best specific growth rate (SGR), feed conversion ratio (FCR) and feed efficiency ratio (FER) were found in nanno 7. Also, it was observed that spiru 7 have a high significant difference (P<0.05) in protein efficiency ratio (PER) and protein productive value (PPV). On the other hand, the results showed that the lowest AWG, ADG and (FER) appeared in spiru 7%. No mortality in nanno7 and spiru 7. Finally, using of nano 5% or spiru 7% enhance growth performance and feeding in tilapia fish.