Pengaruh Kombinasi Magnesium Stearat dan Talkum sebagai Lubrikan terhadap Profil Disolusi Tablet Ibuprofen

A research has been conducted on the combination effect of magnesium stearate and talc as a lubricant to the dissolution profile of Ibuprofen tablets. Of the three formulas made Ibuprofen tablets with wet granulation method. Evaluation of tablets include uniformity of size, weight uniformity, tablet hardness, disintegration, friability of tablets, assay and dissolution. Results of dissolution percentage was done up to 60 indicated that the dissolution the average was 95,72%, 97,65% and 99,93% consecutively for F I, F II and F III. In addition, result of the dissolution efficiency up to 60 minutes for each formula was 82,10%, 87,58%, and 90,76%. According to the dissolution of the data, formula III which had the highest dissolution results compared to formula I and formula II, which likely due to the ratio of the combination of magnesium stearate and talc as a lubricant smaller levels of magnesium stearate.

In Silico Analysis of Curcuma longa Against PCAF Histon Asetiltransferase

Curcumin turmeric (C. longa) has been found capable of inhibiting p300/CBP associated factor (PCAF) histone asetyltransferase (HAT) as a cancer biomarker. However, the molecular mechanism of inhibition is unkown. This study is aimed to analyse the molecular inhibition mechanisms by in silico method. The analysis showed that curcumin’s Gibbs free energy (ΔG) was stable and capable of binding amino acid residues believed to be active site of PCAF HAT. Curcumin is predicted to be a PCAF HAT inhibitor, and further in vitro analysis is required.

Immobilization of Rhizopus oryzae Lipase on Zeolit, CaCO 3 , Silica Gel, and Cow Bone

Food production in Indonesia is constrained by the high cost of lipase that is still imported from abroad. To overcome this problem, research of food production has been conducted using crude extract of lipase produced by indigenous species of fungi Rhizopus oryzae. The R. oryzae is edible indicating that it is safe to be used in the production of food products. Enzymes have an ability to catalyze specific chemical reactions with high efficiency and low energy cost. Enzyme immobilization is a recovery techique that has been studied in several years, using supporting materials as a medium to help enzyme dissolutions to the substrate. Several supporting materials such as zeolit, CaCO 3 , silica gel, and cow bone were selected by its ability to adsorb lipase. CaCO 3 shows enzyme loading rate respectively 99.46%, giving more lipase to adsorb than zeolit (90.69%), cow bone (91.56%), and silica gel (59.63%). In this research, condition factors, such as optimum pH, optimum temperature, and storage ability of the matrix were investigated. Free lipase reacts optimally at pH 7 and temperature 30°C. Identical result showed for lipase in cow bone. Lipase in CaCO 3 reacts optimally at pH 8 and temperature 35°C. Lipase in zeolit and silica gel reacts optimally at pH 8 and temperature 30°C. Half life time estimation was one week in storage condition temperature at 4°C and each optimum pH.

Isolation and Characterization of Endophytic Bacteria from Tembelekan ( Lantana camara L.) as Antibacterial Compounds Producer

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

<i>In Silico</i> Analysis of <i>Curcuma longa</i> Against PCAF Histon Asetiltransferase

Curcumin turmeric (C. longa) has been found capable of inhibiting p300/CBP associated factor (PCAF) histone asetyltransferase (HAT) as a cancer biomarker. However, the molecular mechanism of inhibition is unkown. This study is aimed to analyse the molecular inhibition mechanisms by in silico method. The analysis showed that curcumin’s Gibbs free energy (ΔG) was stable and capable of binding amino acid residues believed to be active site of PCAF HAT. Curcumin is predicted to be a PCAF HAT inhibitor, and further in vitro analysis is required.

Immobilization of <i>Rhizopus oryzae</i> Lipase on Zeolit, CaCO₃, Silica Gel, and Cow Bone

Food production in Indonesia is constrained by the high cost of lipase that is still imported from abroad. To overcome this problem, research of food production has been conducted using crude extract of lipase produced by indigenous species of fungi Rhizopus oryzae. The R. oryzae is edible indicating that it is safe to be used in the production of food products. Enzymes have an ability to catalyze specific chemical reactions with high efficiency and low energy cost. Enzyme immobilization is a recovery techique that has been studied in several years, using supporting materials as a medium to help enzyme dissolutions to the substrate. Several supporting materials such as zeolit, CaCO 3 , silica gel, and cow bone were selected by its ability to adsorb lipase. CaCO 3 shows enzyme loading rate respectively 99.46%, giving more lipase to adsorb than zeolit (90.69%), cow bone (91.56%), and silica gel (59.63%). In this research, condition factors, such as optimum pH, optimum temperature, and storage ability of the matrix were investigated. Free lipase reacts optimally at pH 7 and temperature 30°C. Identical result showed for lipase in cow bone. Lipase in CaCO 3 reacts optimally at pH 8 and temperature 35°C. Lipase in zeolit and silica gel reacts optimally at pH 8 and temperature 30°C. Half life time estimation was one week in storage condition temperature at 4°C and each optimum pH.

Isolation and Characterization of Endophytic Bacteria from Tembelekan (<i>Lantana camara</i> L.) as Antibacterial Compounds Producer

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

Motivasi dan Partisipasi Peternak dalam Pengembangan Ternak Kerbau di Kabupaten Pandeglang (Studi Kasus: Desa Cibarani Kecamatan Cisata) (The Motivation and Participation of Farmers in the Farming Development Buffaloes in Pandeglang District (Case Study : Cibarani Village Cisata Sub-District))

Buffalo’s population decreased for 0.06% every year in 2005 to 2013 in Pandeglang District. Development programs and activities were supported by the local government policy in order to overcome the problems. Farmers as the main human resources play important roles in buffaloes farming development. This research was conducted to assess the knowledge and skills, motivation and participation of farmers in buffaloes farming development in Pandeglang District, using the study case in Cibarani Village, Cisata Sub-district. Surveys and interview to 93 farmers were used to obtain the data required in the study. Farmers were divided into four farmer groups i.e. Saluyu Jaya, Cirukap Makmur, Taruna Mandiri and Putra Makmur with the number of respondents were 16, 10, 21 and 46 respectively. Data were analyzed descriptively and provided in tables. Level of knowledge, motivation and participation of human resources were analyzed using a Mann-Whitney non-parametric statistics. The results showed that the farmer’s knowledge and participation were on satisfactory level, while their motivations, in keeping buffaloes farming as their main activities, were high.Keyword : buffalo, farmer, knowledge, motivation, participation

Exploring the experience of prostate surgery among male patients with benign prostatic hyperplasia: a qualitative content analysis

FORMAL-BASED FRAMEWORK FOR ANALYSIS OF LOGICAL ARCHITECTURE