Journals
2024 EN
Gürsoy Güney · Çiçek Zehra · Tekerek Saniye
+3 more
This study revealed the effective production of nickel oxide nanoparticles (NPs) using chelating agents derived from the leaves of Paulownia tomentosa plants and nickel (II) chloride hexahydrate (NiCl 2 ⋅6H 2 O) as the precursor. Nickel oxide NPs (NiO NPs) have been successfully prepared by a green synthesis method using extracts of P. tomentosa leaves in water. The morphology and crystallinity of the NPs were investigated via X‐ray diffraction (XRD), scanning electron microscopy (SEM), UV–visible spectroscopy (UV–vis), energy‐dispersive X‐ray analysis (EDX), Fourier transform infrared spectroscopy (FT‐IR) and thermogravimetric analysis (TGA). The formation behaviour of NiO NPs and the microstructural and physical properties of the resulting particles were studied. This study additionally investigated the antimicrobial, antibiofilm and cytotoxic effects of NiO NPs. Microstructurally uniform NiO NPs with a nanometre diameter were consistently produced using an eco‐friendly processing approach and exhibited a visible‐range transparency of 89%. The obtained SEM images confirmed the octahedral shape of the NPs. The NiO NPs acquired were found to have potent antimicrobial efficacy against all the other bacterial strains. As a result of their antibiofilm effects on pathogenic Escherichia coli and Pseudomonas aeruginosa bacteria, NiO NPs strongly inhibited these bacteria in a concentration‐dependent manner. In the cytotoxicity study of NiO NPs, a decrease in cell viability was shown to occur depending on the concentration of the cell lines used.
Journals
2024 EN
Kahraman Sevgi · Gungor Ozge · Erkan Sultan
+3 more
In this study, new Cu (II) and Zn (II) chelates ( 3 – 6 ) of 3‐/or 4‐ethyl and azo chromophore groups (NN) containing Schiff base ligands (2‐{( E )‐[(4‐ethylphenyl)imino]methyl}‐4‐[(E)‐phenyldiazenyl]phenol, HL 1 ( 1 ) and 2‐{( E )‐[(3‐ethylphenyl)imino]methyl}‐4‐[(E)‐phenyldiazenyl]phenol, HL 2 ( 2 ) were prepared and characterized by the analytical and spectroscopic methods. Crystal structures of chelates 3 , 4 and 6 were determined by single‐crystal X‐ray diffraction studies. The ligands behaved as bidentates, coordinating through the nitrogen atom of the azomethine group (CHN) and the oxygen atom of the α‐hydroxyl group (OH). The metal:ligand ratio in the prepared copper (II) and zinc (II) chelates was found to be 1:2 by analytical and spectral analysis. The bands in the infrared spectra of the metal chelates in the region of 522–504 cm −1 and 467–455 cm −1 are attributed to ν(MO) and ν(MN) vibrations, respectively. When the DNA binding activities of the compounds were examined, it was determined that compound 4 had the highest binding constant. Fluorescence and viscosity data showed that the compounds interact with DNA through minor grove binding mode. Furthermore, anticancer properties of the compounds were investigated. Ligand 2 was found to be highly effective against the HepG2 cell with an IC 50 of 0.31 μM. In addition, structural analysis, frontier molecular orbitals, molecular electrostatic potential maps, Hirshfeld surface analysis of the synthesized compounds using computational chemistry methods and anticancer activity studies were carried out by molecular docking.
Journals
2024 EN
Gurung Dinesh · Murad Md Shafinur · Asmatulu Eylem
+4 more
Polyether ether ketone (PEEK) sulfonation process is a reasonably easy but time‐consuming to manufacture composites. This study focuses on altering PEEK powder in a sulfuric acid solution via a sulfonation process to develop sulfonated PEEK (SPEEK) resin for the fiber‐reinforced thermoplastic composite manufacturing and characterization steps. In this study, about 5 wt% PEEK powder was sulfonated in 98% sulfuric acid solution for 12 h at 65°C. The water bath technique was used for the precipitation process to obtain the SPEEK polymer. After proper washing and drying, the SPEEK matrix (resin) was obtained by dissolving it in dimethylformamide in a ratio of 1:2. This resin solution was then used to manufacture thermoplastic Kevlar and glass fiber‐reinforced composites employing wet layup process and cured at an elevated temperature under pressure. These SPEEK composites passed the flame retardancy UL94 test with a V0 rating. The average water contact angle of the glass fiber‐ and Kevlar‐SPEEK composites is 93.67° and 102.24°, respectively. The average tensile strength values of these composites were found 222.22 and 284.35 MPa, correspondingly. The smoke density and toxicity tests of the glass fiber‐SPEEK composite confirmed lower smoke generation with the modifications. These SPEEK‐fiber composites can be considered for various industrial applications, including aviation, energy, drone, defense, and automotive.
Journals
2024 EN
Evren Asaf E. · Nuha Demokrat · Özkan Begüm N. S.
+3 more
Abstract This study examines the synthesis and evaluation of 11 newly developed compounds as potential anti‐Alzheimer's agents that occur via cholinesterase and β‐secretase inhibition. The compounds were tested for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) using the modified Ellman method. The results showed that several compounds exhibited significant inhibition of AChE, particularly compounds 6d , 7a , and 7e , which demonstrated high inhibitory activity at lower concentrations, with IC 50 values of 0.120, 0.039, and 0.063 µM, respectively. However, the compounds showed limited effectiveness against BChE, with only a few compounds exhibiting moderate inhibition. Compound 7e showed an inhibitory effect against BACE‐1 close to that of the standard drug. Structural analysis revealed that the compounds with substituted benzothiazole and thiazole moieties exhibited the most promising inhibitory activity. This study provides valuable insights into the potential of these synthesized derivatives as a treatment against Alzheimer's disease. Moreover, the structure, stability, and properties of the active compounds were further investigated using density functional theory calculations. As a final note, the utilization of molecular docking and molecular dynamics simulation studies allowed us to elucidate the action mechanism of the active compounds and gain insights into the structure–activity relationship against AChE and β‐secretase proteins. These computational techniques provide valuable information on the binding modes, interactions with target enzymes, dynamic behavior, and conformational changes of the compounds, enabling a comprehensive understanding of their biological activity.
Journals
2024 EN
Bulkurcuoğlu Bünyamin · Gürbüz Mustafa Ulvi · Tyciakova Silvia
+5 more
ABSTRACT A promising approach to treat colorectal cancer (CRC) involves combining chemotherapy, epigenetics, and gene therapy to combat drug resistance. Multifunctional nanocarriers have emerged as a valuable tool for targeted CRC therapy. By delivering multiple treatments directly to cancer cells, these nanocarriers offer the potential for improved outcomes and reduced side effects. PAMAM‐based dendrimers were functionalized with a unique combination of folic acid, 5‐FU, SAHA, and plasmid DNA pCIneoGFP for targeted delivery to CRC cells. Biophysical characterizations of therapeutic loaded dendrimers and their complexes with pCIneoGFP were performed by: dynamic light scattering, fluorescence spectroscopy, and gel electrophoresis. Further, cellular analyses of dendriplexes demonstrated high transfection efficiency and anticancer activity on HCT 116 and HT‐29 cell lines. We have successfully developed a multifunctional nanocarrier platform based on PAMAM dendrimers, offering a promising tool for targeted combination therapy of CRC.
Journals
2024 EN
Hajebrahimi Farzin · Budak Miray · Saricaoglu Mevhibe
+5 more
Cognitive impairment (CI) is a significant non‐motor symptoms in Parkinson's disease (PD) that often precedes the emergence of motor symptoms by several years. Patients with PD hypothetically progress from stages without CI (PD‐normal cognition [NC]) to stages with Mild CI (PD‐MCI) and PD dementia (PDD). CI symptoms in PD are linked to different brain regions and neural pathways, in addition to being the result of dysfunctional subcortical regions. However, it is still unknown how functional dysregulation correlates to progression during the CI. Neuroimaging techniques hold promise in discriminating CI stages of PD and further contribute to the biomarker formation of CI in PD. In this study, we explore disparities in the clinical assessments and resting‐state functional connectivity (FC) among three CI stages of PD. Methods We enrolled 88 patients with PD and 26 healthy controls (HC) for a cross sectional clinical study and performed intra‐ and inter‐network FC analysis in conjunction with comprehensive clinical cognitive assessment. Results Our findings underscore the significance of several neural networks, namely, the default mode network (DMN), frontoparietal network (FPN), dorsal attention network, and visual network (VN) and their inter–intra‐network FC in differentiating between PD‐MCI and PDD. Additionally, our results showed the importance of sensory motor network, VN, DMN, and salience network (SN) in the discriminating PD‐NC from PDD. Finally, in comparison to HC, we found DMN, FPN, VN, and SN as pivotal networks for further differential diagnosis of CI stages of PD. Conclusion We propose that resting‐state networks (RSN) can be a discriminating factor in distinguishing the CI stages of PD and progressing from PD‐NC to MCI or PDD. The integration of clinical and neuroimaging data may enhance the early detection of PD in clinical settings and potentially prevent the disease from advancing to more severe stages.
Journals
2024 EN
Bal Sezen · Sarıkaş Ali · Yayla Ayşe
+5 more
Abstract Engineering can enhance students' theoretical knowledge and practical application skills when integrated into K‐12 education. This approach encourages students to contemplate technology and engineering in more depth. Field programmable gate array (FPGA) technology training is becoming increasingly important, especially for vocational high school technical teachers. This study focuses on providing FPGA technology training to these vocational and technical teachers. The aim is to improve their theoretical understanding of FPGA digital design and practical application skills. By incorporating the latest technology into their courses, educators can offer students in‐depth learning and practical experience in electronic engineering and digital design. The training program covered the basics of FPGA, its applications, and how it can benefit vocational high schools in laboratory environments. A total of 34 technical teachers participated in the training program. The effectiveness of the program was evaluated through pre‐ and posttraining questionnaires. The results showed that most of the questionnaire items were statistically and practically significant and varied according to the teachers' department, teaching experience, and level of education.
Journals
2024 EN
Uzun Fatih · Güner Ahmet · Demirci Gökhan
+16 more
Abstract Background Double kissing (DK)‐crush and T‐stenting and small protrusion (TAP) techniques are gaining popularity, but the comparison for both techniques is still lacking. This study sought to retrospectively evaluate the long‐term outcomes of DK‐crush and TAP techniques in patients with complex bifurcation lesions. Methods A total of 255 (male: 205 [80.3%], mean age: 59.56 ± 10.13 years) patients who underwent coronary bifurcation intervention at a single‐center between January 2014 and May 2021 were included. Angiographic features, procedure details, and in‐hospital or long‐term outcomes were assessed. The primary endpoint was target lesion failure (TLF), defined as the combination of cardiac death, target vessel myocardial infarction, or clinically driven‐target lesion revascularization (TLR). The regression models were adjusted applying by the inverse probability weighted (IPW) approach to reduce treatment selection bias. Results The initial management strategy was DK‐crush in 152 (59.6%) patients and TAP in 103 (40.4%) cases. The SYNTAX scores (24.58 ± 7.4 vs. 24.26 ± 6.39, p = 0.846) were similar in both groups. The number of balloon (6.32 ± 1.82 vs. 3.92 ± 1.19, p < 0.001) usage was significantly higher in the DK‐crush group than in the TAP group. The rates of TLF (11.8 vs. 22.3%, p = 0.025) and clinically driven TLR (6.6 vs. 15.5%, p = 0.020) were significantly lower in the DK‐crush group compared to the TAP group. The long‐term TLF was significantly higher in the TAP group compared to the DK‐crush group (unadjusted HR: 1.974, [95% CI: 1.044–3.732], p = 0.035 and adjusted HR [IPW]: 2.498 [95% CI: 1.232–5.061], p = 0.011). Conclusion The present study showed that the DK‐crush technique of bifurcation treatment was associated with lower long‐term TLF and TLR rates compared to the TAP technique.
Journals
2024 EN
Hakgor Aykun · Dursun Atakan · Kahraman Basak Catalbas
+6 more
Abstract Background Pulmonary hypertension (PH) and right ventricular dysfunction are poor prognostic predictors in patients underwent transcatheter aortic valve implantation (TAVI) for severe aortic stenosis (AS). Aims The prognostic impact of the main pulmonary artery/ascending aorta diameter ratio (MPA/AOr), measured simply by computed‐tomographic angiography (CTA), was investigated in this patient group. Methods A total of 374 retrospectively evaluated patients (mean age 78.1 ± 8.4 years, 192 [51.3%] females) who underwent TAVI for severe AS were included. MPA/AOr was measured on preprocedural CTA in all patients and the effect of this measurement on the presence of PH, in‐hospital and 2‐year‐overall long‐term mortality was investigated. Results The presence of PH was defined as a systolic pulmonary artery pressure (sPAP) >42 mmHg measured by echocardiography. According to multivariate‐logistic‐regression analysis, MPA/AOr (adjusted [Adj] odds ratio [OR]: 1.188, confidence interval [CI] 95% [1.002–1.410], p = 0.048), tricuspid annular plane systolic excursion (TAPSE) (adj OR:0.736, CI 95% [0.663–0.816], p < 0.001) and left atrial diameter (adj OR:1.051, CI 95% [1.007–1.098], p = 0.024) were identified as independent predictors of PH. In addition, a statistically significant correlation was found between MPA/AOr and TAPSE ( r : −0.283, p < 0.001). Furthermore, MPA/AOr was found to be an independent predictor of both in‐hospital (adj OR:1.434, CI 95% [1.093–1.881], p = 0.009) and 2‐year long‐term (adj OR:1.518, CI 95% [1.243–1.853], p < 0.001) mortality in multivariate analysis including TAPSE, STS score and sPAP. In the 2‐year Kaplan–Meier survival probability analysis, an MPA/AOr >0.86 was found to have a hazard ratio of 3.697 (95% CI: 2.341–5.840), with a log‐rank p < 0.001. Conclusion MPA/AOr, which can be measured simply by CTA, may be useful as an indicator of the presence of PH and poor prognosis in patients planned for TAVI for severe AS.
Journals
2024 EN
Çizgici Ahmet Yaşar · Güner Ahmet · Alizade Elnur
+18 more
Abstract Background Double kissing crush (DKC) and nano‐crush (NC) techniques are frequently used, but the comparison for both techniques is still lacking. The goal of this multicenter study was to retrospectively assess the midterm clinical results of DKC and NC stenting in patients with complex bifurcation lesions (CBLs). Methods A total of 324 consecutive patients [male: 245 (75.6%), mean age: 60.73 ± 10.21 years] who underwent bifurcation percutaneous coronary intervention between January 2019 and May 2023 were included. The primary endpoint defined as the major cardiovascular events (MACE) included cardiac death, target vessel myocardial infarction (TVMI), or clinically driven target lesion revascularization (TLR). Inverse probability weighting (IPW) was performed to reduce treatment selection bias. This is the first report comparing the clinical outcomes of DKC and NC stenting in patients with CBL. Results The initial revascularization strategy was DKC in 216 (66.7%) cases and NC in 108 (33.3%) patients. SYNTAX scores [25.5 ± 6.73 vs. 23.32 ± 6.22, p = 0.005] were notably higher in the NC group than the DKC group. The procedure time (76.98 ± 25.1 vs. 57.5 ± 22.99 min, p = 0.001) was notably higher in the DKC group. The incidence of MACE (18.5 vs. 9.7%, p = 0.025), clinically driven TLR (14.8 vs. 6%, p = 0.009), and TVMI (10.2 vs. 4.2%, p = 0.048) were notably higher in the NC group than in the DKC group. The midterm MACE rate in the overall population notably differed between the NC group and the DKC group (adjusted HR (IPW): 2.712, [95% CI: 1.407−5.228], p = 0.003). Conclusion In patients with CBLs, applying the DKC technique for bifurcation treatment had better ischemia‐driven outcomes than the NC technique.