Single-particle cryo-EM of macromolecular complexes at near-atomic resolution

Eugene B. Pichkur1, Pavel Kasatsky2, Timur N. Baymukhametov1, Yury M. Chesnokov1, Mikhail Y. Presniakov1, Alexander Miasnikov3, Andrey L. Konevega2, Alexander L. Vasiliev1, Mikhail V. Kovalchuk1 1NRC Kurchatov Institute, Moscow, Russia, Moscow, Russian Federation, 2National Research Centre “Kurchatov Institute”, Petersburg Nuclear Physics Institute, Gatchina, Russia, Gatchina, Russian Federation, 3University of California San Francisco, Department of Biochemistry and Biophysic, San Francisco, United States E-mail: [email protected]

Electron microscopy methods in studies of archaeological objects

Study of spatial structure TvNiR by cryo-TEM

Cryo-electron microscopy of biological objects is one of the most interesting and promising trends now. A distinctive feature of this method is the opportunity to explore biological objects in their native state, without conducting any preliminary preparatory actions. Single particle analysis (SPA) method is used for the study of biological objects in small sizes, such as viruses, membrane proteins, etc. In this paper, an example of the hexamer cytochrome c nitrite reductase acquisition of thioalkalivibrio nitratireducens (TVNiR) [1] shows the capabilities of modern structural biology. The cryo-TEM specimens were prepared in native buffer conditions: samples were applied onto the grid manually via the side port of the Vitrobot (FEI, US) directly onto the Lacey carbon-coated side of the 300-mesh copper TEM grid using a pipette. After the sample deposition the grid was blotted and immediately plunged into the ethane at liquid N2 temperature. All samples were studied in bright field (BF) TEM in a Titan Krios 60-300 TEM/STEM (FEI, Oregon, US) equipped with a spherical aberration (Cs) corrector (image corrector), a direct detection camera Falcon II (FEI, US) and post-column energy filter (Gatan, Pleasanton, CA, US). In the present investigation the TEM was operated at 300 kV. The micrographs were obtained in Low Dose mode with total electron dose of less than 60e/Å2. Digitral Micrograph (Gatan, US) and TIA (FEI,US) software were used for the image processing.

Tc-enhancement of Fe1+δSe by electrochemical lithium intercalation

Evgeny Antipov1, Anastasya Alekseeva2, Oleg Drozhzhin2, Olga Volkova1, Alexander Vasiliev3, Dmitry Chareev4, Juri Grin5 1Department of Chemistry, Lomonosov Moscow State University, Moscow, Russian Federation, 2MPG-MSU Partner Group, Department of Chemistry, Lomonosov Moscow State University, Moscow, Russian Federation, 3Department of Physics, Lomonosov Moscow State University, Moscow, Russian Federation, 4Institute of Experimental Mineralogy, Russian Academy of Sciences, Chernogolovka, Russian Federation, 5Max-Planck-Institut für Chemische Physik fester Stoffe, Dresden, Germany E-mail: [email protected]

Structure of a trinuclear μ3-vinylidene cluster CpMnFePt(μ3-C=CHPh)(PPh3)(CO)6

Erratum to: “Thin film negative electrode based on silicon composite for lithium-ion batteries”

AB0531 Anti-centromere positive primary sjÖgren's syndrome – challenge to classification criteria

Analysis of pion production data measured by HADES in proton-proton collisions at 1.25 GeV

Baryon resonance production in proton-proton collisions at a kinetic beamenergy of 1.25 GeV is investigated. The multi-differential data were measuredby the HADES collaboration. Exclusive channels with one pion in the final state($np\pi^{+}$ and $pp\pi^{0}$) were put to extended studies based on variousobservables in the framework of a one-pion exchange model and with solutionsobtained within the framework of a partial wave analysis (PWA) of theBonn-Gatchina group. The results of the PWA confirm the dominant contributionof the $\Delta$(1232), yet with a sizable impact of the $N$(1440) andnon-resonant partial waves.

Erratum to: Production of $${\pi ^0}$$ π 0 and $$\eta $$ η mesons up to high transverse momentum in pp collisions at 2.76 TeV

The invariant differential cross sections for inclusive π0 and η mesons at midrapidity were measured in pp collisions at s√=2.76 TeV for transverse momenta 0.4<pT<40 GeV/c and 0.6<pT<20 GeV/c, respectively, using the ALICE detector. This large range in pT was achieved by combining various analysis techniques and different triggers involving the electromagnetic calorimeter (EMCal). In particular, a new single-cluster, shower-shape based method was developed for the identification of high- pT neutral pions, which exploits that the showers originating from their decay photons overlap in the EMCal. Above 4 GeV/ c , the measured cross sections are found to exhibit a similar power-law behavior with an exponent of about 6.3. Next-to-leading-order perturbative QCD calculations differ from the measured cross sections by about 30% for the π0 , and between 30-50% for the η meson, while generator-level simulations with PYTHIA 8.2 describe the data to better than 10-30%, except at pT<1 GeV/ c . The new data can therefore be used to further improve the theoretical description of π0 and η meson production

Development of $^{100}$Mo-containing scintillating bolometers for a high-sensitivity neutrinoless double-beta decay search

This paper reports on the development of a technology involving$^{100}$Mo-enriched scintillating bolometers, compatible with the goals ofCUPID, a proposed next-generation bolometric experiment to search forneutrinoless double-beta decay. Large mass ($\sim$1~kg), high optical quality,radiopure $^{100}$Mo-containing zinc and lithium molybdate crystals have beenproduced and used to develop high performance single detector modules based on0.2--0.4~kg scintillating bolometers. In particular, the energy resolution ofthe lithium molybdate detectors near the $Q$-value of the double-betatransition of $^{100}$Mo (3034~keV) is 4--6~keV FWHM. The rejection of the$\alpha$-induced dominant background above 2.6~MeV is better than 8$\sigma$.Less than 10~$\mu$Bq/kg activity of $^{232}$Th ($^{228}$Th) and $^{226}$Ra inthe crystals is ensured by boule recrystallization. The potential of$^{100}$Mo-enriched scintillating bolometers to perform high sensitivitydouble-beta decay searches has been demonstrated with only 10~kg$\times$dexposure: the two neutrino double-beta decay half-life of $^{100}$Mo has beenmeasured with the up-to-date highest accuracy as $T_{1/2}$ = [6.90 $\pm$0.15(stat.) $\pm$ 0.37(syst.)] $\times$ 10$^{18}$~yr. Both crystallization anddetector technologies favor lithium molybdate, which has been selected for theongoing construction of the CUPID-0/Mo demonstrator, containing several kg of$^{100}$Mo.